MLST 1.8 (MultiLocus Sequence Typing)

Select MLST configuration
Achromobacter spp. Acinetobacter baumannii#1 Acinetobacter baumannii#2 Aeromonas spp. Anaplasma phagocytophilum Arcobacter spp. Aspergillus fumigatus Bacillus cereus Bacillus licheniformis Bacillus subtilis Bartonella henselae Bordetella spp. Borrelia spp. Brachyspira hampsonii Brachyspira hyodysenteriae Brachyspira intermedia Brachyspira pilosicoli Brachyspira spp. Brucella spp. Burkholderia cepacia complex Burkholderia pseudomallei Campylobacter concisus/curvus Campylobacter fetus Campylobacter helveticus Campylobacter hyointestinalis Campylobacter insulaenigrae Campylobacter jejuni Campylobacter lanienae Campylobacter lari Campylobacter sputorum Campylobacter upsaliensis Candida albicans Candida glabrata Candida krusei Candida tropicalis Carnobacterium maltaromaticum Chlamydiales spp. Citrobacter freundii Clonorchis sinensis Clostridium botulinum Clostridium difficile Clostridium septicum Corynebacterium diphtheriae Cronobacter spp. Enterobacter cloacae Enterococcus faecalis Enterococcus faecium Escherichia coli#1 Escherichia coli#2 Flavobacterium psychrophilum Haemophilus influenzae Haemophilus parasuis Helicobacter cinaedi Helicobacter pylori Helicobacter suis Kingella kingae Klebsiella oxytoca Klebsiella pneumoniae Kudoa septempunctata Lactobacillus salivarius Lactococcus lactis Leptospira spp. Leptospira spp.#2 Leptospira spp.#3 Listeria Listeria monocytogenes Macrococcus canis Macrococcus caseolyticus Mannheimia haemolytica Melissococcus plutonius Moraxella catarrhalis Mycobacterium abscessus Mycobacterium massiliense Mycoplasma agalactiae Mycoplasma bovis Mycoplasma hyopneumoniae Mycoplasma hyorhinis Mycoplasma iowae Mycoplasma pneumoniae Mycoplasma synoviae Neisseria spp. Orientia tsutsugamushi Ornithobacterium rhinotracheale Paenibacillus larvae Pasteurella multocida#1 Pasteurella multocida#2 Pediococcus pentosaceus Photobacterium damselae Porphyromonas gingivalis Propionibacterium acnes Pseudomonas aeruginosa Pseudomonas fluorescens Riemerella anatipestifer Salmonella enterica Sinorhizobium spp. Staphylococcus aureus Staphylococcus epidermidis Staphylococcus haemolyticus Staphylococcus lugdunensis Staphylococcus pseudintermedius Stapylococcus hominis Stenotrophomonas maltophilia Streptococcus agalactiae Streptococcus bovis/equinus complex (SBSEC) Streptococcus canis Streptococcus dysgalactiae equisimilis Streptococcus gallolyticus Streptococcus oralis Streptococcus pneumoniae Streptococcus pyogenes Streptococcus suis Streptococcus thermophilus Streptococcus thermophilus#2 Streptococcus uberis Streptococcus zooepidemicus Streptomyces spp Taylorella spp. Tenacibaculum spp. Trichomonas vaginalis Vibrio cholerae Vibrio parahaemolyticus Vibrio spp. Vibrio tapetis Vibrio vulnificus Wolbachia Xylella fastidiosa Yersinia pseudotuberculosis Yersinia ruckeri Yersinia spp.

Select type of your reads
Assembled Genome/Contigs* 454 - single end reads 454 - paired end reads Illumina - single end reads Illumina - paired end reads Ion Torrent SOLiD - single end reads SOLiD - mate pair reads

MLST configuration

• Acinetobacter baumannii (Acinetobacter baumannii #1 [1], Acinetobacter baumannii #2 (link)).
• Escherichia coli (Escherichia coli #1 [4], Escherichia coli #2 [5]).
• Pasteurella multocida (Pasteurella multocida #1 (RIRDC), Pasteurella multocida #2 (multihost)).
• Leptospira (Leptospira #1, Leptospira #2, Leptospira #3).

REFERENCES

1. Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodriguez-Valera F. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 2005; 43:4382-90.
2. Griffiths D, Fawley W, Kachrimanidou M, et al. Multilocus sequence typing of Clostridium difficile. J Clin Microbiol 2010; 48:770-8.
3. Lemee L, Dhalluin A, Pestel-Caron M, Lemeland JF, Pons JL. Multilocus sequence typing analysis of human and animal Clostridium difficile isolates of various toxigenic types. J Clin Microbiol 2004; 42:2609-17.
4. Wirth T, Falush D, Lan R, et al. Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol 2006; 60:1136-51.
5. Jaureguy F, Landraud L, Passet V, et al. Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains. BMC Genomics 2008; 9:560.

CITATIONS

For publication of results, please cite:

• Multilocus Sequence Typing of Total Genome Sequenced Bacteria.
  Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H, Marvig RL, Jelsbak L, Sicheritz-Pontén T, Ussery DW, Aarestrup FM and Lund O.
  J. Clin. Micobiol. 2012. 50(4): 1355-1361.
  PMID: 22238442         doi: 10.1128/JCM.06094-11